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Transcription and Translation Reagents

A variety of kits, systems, buffers, inhibitors, RNA cap analogs, reagents, and solutions for use in experiments involving transcription and translation. Products can be used in the synthesis of RNA probes.
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31 – 45 of 164 results
31

Invitrogen™ Cap Analog (m7G(5')ppp(5')G), 25 A254 Units

For 5' capped RNA molecule synthesis as part of in vitro transcription reactions

ENCOMPASS_PREFERRED
32

R&D Systems™ Mouse Mesenchymal Stem Cell Functional Identification Kit

To verify multipotency of mouse mesenchymal stem cells by in vitro functional differentiation

33

Promega Wheat Germ Extract

Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) and is used for in vitro translation..

34

Promega TNT™ T7 Quick for PCR DNA

TNT T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum protein expression from PCR templates.

35

Promega Transcend™ Nonradioactive Translation Detection Systems: IRNA

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

36

Promega TNT™ T7 Quick Starter Bundle, Colorimetric

Purchase this bundle, and get the TNT T7 Quick Coupled Transcription/Translation System, your choice of Transcend Translation Detection System, and receive two cell-free expression-qualified expression vectors, pTNT and pCMVTNT Vectors, at no extra cost.

37

Promega TNT™ Coupled Reticulocyte Lysate Systems

TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Content And Storage <-65°C
For Use With (Application) GST pull-downs, Gel shift assays, co-immunoprecipitation, characterization of Protein modifications, Protein-RNA interactions, Protein activity studies, confirmation of gene products, Protein truncation test (PTT)
38

Promega Transcend™ Nonradioactive Translation Detection Systems

Non-Radioactive Translation Detection Systems for colorimetric or chemiluminescent detection of proteins synthesized in vitro. Biotinylated proteins are detected using Streptavidin-Alkaline Phosphatase or Streptavidin-HRP.

39

Promega TNT™ Coupled Wheat Germ Extract Systems

The TNT Coupled Wheat Germ Extract Systems are one-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.

Includes 1mL TNT™ Wheat Germ Extract, 90μL TNT™ Reaction Buffer, 60μL TNT™ RNA Polymerase (SP6, T3 or T7, individually or 2 x 30μL when two Polymerases are
No. of Reactions 40
Content And Storage <-65°C
For Use With (Application) Translation of mammalian transcription factors
40

Promega Recombinant RNasin™ Ribonuclease Inhibitor

Ribonuclease inhibitors with broad-spectrum RNase inhibitory properties. Recombinant and native available. Plus version protects RNA before, during and after heating.

Content And Storage -30°C to -10°C
Concentration 20 to 40 U/μL
For Use With (Application) RT-PCR, protection of mRNA in cDNA synthesis reactions, in-vitro transcription/translation, Increase yield and activity of polysomes, improvement of in-vitro virus replication, improvement of RNA translation in homologous systems
41

Promega Riboprobe™ Systems

The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.

Content And Storage Store at -20°C.
Quantity 1 System
For Use With (Application) Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
42

Promega Ribo m7G Cap Analog

This methylated ribonucleotide can be incorporated onto the 5'-end of in vitro transcripts and simulates the cap structure found on most eukaryotic mRNA molecules.

Content And Storage -30°C to -10°C
For Use With (Application) Enhances translation efficiency in many reticulocyte-based reactions and protects RNA from intracellular digestion
43

Promega RiboMAX™ Large Scale RNA Production Systems

Designed for large-scale RNA production, the RiboMAX Systems consistently produce 2-5mg RNA from a 1ml reaction volume.

Includes Linear control DNA, RQ1 RNase-free DNase, Nuclease-free Water, Enzyme mix, rATP, rCTP, rGTP, rUTP, Sodium Acetate, 3m (pH 5.2), 5X Transcription Buffer
Content And Storage -30°C to -10°C
Quantity 1 System
For Use With (Application) Produce 2 to 5mg/mL of RNA in a 1mL reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe™ system transcription reaction
44

Promega Riboprobe™ Combination Systems

The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.

Content And Storage -30°C to -10°C
Quantity 1 System
For Use With (Application) Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
45

Promega E. coli S30 Extract System for Linear Templates

The E. coli S30 Extract System for Linear Templates allows transcription/translation of linear DNA templates.